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| Laboratory Tips Safety proceedures, test reagents, drilling rubber stoppers, bending glass tubes, etc. Contributed to by chemists. |
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# 1

2008-08-26, 10:38
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Micron filter size... too small?
Have googled the following question but couldnt find any answers so...
My friend has got hold of 0.10 micron filters instead of his usual 0.22 micron. What he is wondering is that will this size be too small in that it'll actually filter out some of his end product too??? and not just the nasties such as bacteria and viri?
what he wants to let through is this : http://en.wikipedia.org/wiki/Trenbolone
will that molecule pass through such a small filter unscathed?
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# 2

2008-08-26, 12:06
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Re: Micron filter size... too small?
If you are filtering particulates out of a solution of your desired compound, the pore size becomes irrelevant...
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# 3

2008-08-26, 13:19
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Re: Micron filter size... too small?
Quote:
Originally Posted by fcknut
If you are filtering particulates out of a solution of your desired compound, the pore size becomes irrelevant...
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Not neccisarily true...
...this nigga got a few orders of magnitude, though...
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# 4

2008-08-26, 14:20
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Re: Micron filter size... too small?
Quote:
Originally Posted by JoePedo
Not neccisarily true...
...this nigga got a few orders of magnitude, though...
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which is to say necessarily in B4 sofh
hope that helps
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# 5

2008-08-26, 20:03
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Re: Micron filter size... too small?
Quote:
Originally Posted by gigster
which is to say necessarily in B4 sofh
hope that helps
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what lol???
non comprendhe!
hey greeko!!
but this >
"If you are filtering particulates out of a solution of your desired compound, the pore size becomes irrelevant... "
is very educational to a thicko like me, i even understand the answer lol, thank you Sir.
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# 6

2008-08-27, 08:54
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Re: Micron filter size... too small?
es comprender o no entender claro ahoha? bueno.............................but I understand what your saying and Im cool with that so whats the ? again?
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# 7

2008-08-27, 09:30
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Re: Micron filter size... too small?
Quote:
Originally Posted by JoePedo
Not neccisarily true...
...this nigga got a few orders of magnitude, though...
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Well yes indeed! But I think we're safe with steroids and micron filters...
Quote:
Originally Posted by LSDPanic
is very educational to a thicko like me, i even understand the answer lol, thank you Sir.
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Oh good! Glad to be of assistance...!
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# 8

2008-08-27, 12:59
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Re: Micron filter size... too small?
Quote:
Originally Posted by gigster
which is to say necessarily in B4 sofh
hope that helps
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fuck. 
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# 9

2008-08-27, 13:11
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Re: Micron filter size... too small?
Quote:
Originally Posted by LSDPanic
what lol???
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We always send Foreman out to harvest us some oxycotton before tackling srs fking bsns.
Helps keep the gimp and the autistic asshole from killing each other when drawing reaction schema on the whiteboard.
Quote:
Originally Posted by fcknut
Well yes indeed! But I think we're safe with steroids and micron filters...
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Yeah.  It's stuffing macropeptides through an angstrom-scale lattice that's a real bitch.
...though I'd probably prefilter a suspension a few times before even trying to stuff it through a micron filter.
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# 10

2008-08-28, 00:22
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Regular
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Aloha
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Re: Micron filter size... too small?
Quote:
Originally Posted by JoePedo
We always send Foreman out to harvest us some oxycotton before tackling srs fking bsns.
Helps keep the gimp and the autistic asshole from killing each other when drawing reaction schema on the whiteboard.
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...lulwut?
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#11
 2008-08-28, 03:11
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Regular
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Anacortes, Washington, US
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Re: Micron filter size... too small?
THIS THREAD IS BEING EATEN BY MEXICANS.
Not that I have a problem with that.
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#12
 2008-08-28, 03:48
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Re: Micron filter size... too small?
Quote:
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Originally Posted by LSDPanic
Have googled the following question but couldnt find any answers so...
My friend has got hold of 0.10 micron filters instead of his usual 0.22 micron. What he is wondering is that will this size be too small in that it'll actually filter out some of his end product too??? and not just the nasties such as bacteria and viri?
what he wants to let through is this : http://en.wikipedia.org/wiki/Trenbolone
will that molecule pass through such a small filter unscathed?
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Okay. I had to click the link to see what you are talking about. Its molecular mass is 270.37. Peptides are filtered through smaller pores than 0.10 um filters. Peptides are in the molecular mass of the thousands. What you are talking about here only constitutes the size of a moderately sized monomer. I mean look at the structure of it. You are talking about bonds on the size of E-10 (angstroms). The filter in question is E-6 (micron). Unscathed as long as you keep it in a solution that its remains stable in... sure.
But if you want to filter out... virii and bacteria (WTF?)... a filter like that is not going to do the work. You either need to combine that steroid with an acid/base to make a salt and take it out of solution or just conduct your synthesis in a sterile environment (Ex: laminar flow hood with a UV light). Since it is so small, you could just HPLC or GC it. It should come out relatively quick since it is so much smaller than full on bacterium. If you were just to start the separation out with 100 % H2O with a very slow gradient up to 30 % Acetonitrile, that shit should come out fairly quick while the rest of the crap in solution hasn't even solubilized yet.
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